本文主要研究内容
作者崔娜（2019）在《微藻降解废水中间甲酚的研究及应用》一文中研究指出：间甲酚作为苯酚的甲基化衍生物,是酚类化合物中的代表性物质,因具有较高毒性和腐蚀性被列为我国优先控制污染物之一。间甲酚比苯酚具有更高的毒性,在较低浓度时便可对人类和环境造成危害。利用微藻去除废水中的酚类化合物具有快速、稳定、效率高、能耗低等优点,它可以通过生物富集、生物降解等方式将废水中的酚类化合物或其他难降解有机物转化为无毒物质,从而达到化工工业废水的排放要求。本课题以人工配制的间甲酚模拟废水为研究对象,研究了间甲酚生物降解的优势藻种及初始藻密度、初始培养基pH值和初始间甲酚浓度对微藻生物降解间甲酚的影响;比较了蛋白核小球藻驯化前后对间甲酚耐受性和去除效率的变化;探究了蛋白核小球藻对间甲酚的代谢途径;并设计、搭建了藻-膜反应器,考察了间甲酚进水浓度和水力停留时间对反应器运行的影响。得出结论如下:（1）选取对酚类物质有较好降解能力的四种微藻:铜绿微囊藻、蛋白核小球藻、斜生栅藻和鱼腥藻,分别对间甲酚进行生物降解,观察四种微藻对间甲酚的耐受性及降解能力,发现蛋白核小球藻对间甲酚的降解效率最佳;探讨了不同初始藻密度(OD600=0.2、0.4、0.6、0.8、1.0)、不同初始pH值（pH=5.0、6.0、7.0、8.0、9.0）及不同初始间甲酚浓度（100 mg/L、200 mg/L、300 mg/L和400 mg/L）下蛋白核小球藻的生长状况及其对间甲酚的去除效率,发现当蛋白核小球藻的初始藻密度OD600=0.8时,间甲酚的存在对蛋白核小球藻的生长具有明显的促进作用,且4 d内便可将100 mg/L的间甲酚完全降解;培养基初始pH=7.0时,第6 d藻密度为初始藻密度的1.79倍,对间甲酚的去除率达到100%,而酸性条件下的间甲酚去除率仅为28.08%;间甲酚初始浓度对蛋白核小球藻的生长有很大的影响,间甲酚初始浓度越高,对蛋白核小球藻的毒性作用越强,蛋白核小球藻生长及对间甲酚降解的适应期越长。通过一系列的实验,最终确定蛋白核小球藻降解间甲酚的最适初始藻密度为OD600=0.8,最适培养基初始pH=7.0,最适间甲酚初始浓度为100 mg/L。（2）研究了藻种驯化对蛋白核小球藻的生长及对间甲酚降解能力的影响。结果表明,驯化后蛋白核小球藻的适应期缩短,藻密度和叶绿素a含量明显提高,300 mg/L和400 mg/L间甲酚浓度胁迫下的藻体叶绿素a含量分别是未驯化藻体的1.98倍和3.98倍;驯化后藻体对间甲酚的耐受性增加,间甲酚的最大致死浓度由500 mg/L上升到了600mg/L;驯化后蛋白核小球藻在35天内可完全降解400 mg/L的间甲酚,表明藻种驯化可以有效提高微藻的降解能力。（3）探讨了蛋白核小球藻对间甲酚的代谢途径,利用FT-IR和GC-MS的定性分析功能,检测到间甲酚代谢过程中有3-羟基苯甲酸和龙胆酸等中间产物的生成,推测间甲酚可能是通过龙胆酸途径进行降解;间甲酚首先在苯酚羟化酶的作用下代谢形成3-羟基苯甲酸,然后进入龙胆酸途径进一步裂解,最终形成丙酮酸或延胡索酸进入糖酵解（Glycolysis）和三羧酸循环（TCA）。（4）设计、搭建了藻-膜反应器并对间甲酚进水浓度及水力停留时间对藻-膜反应器处理效果的影响进行了研究。结果表明,间甲酚及COD的去除效率随间甲酚进水浓度的升高而降低,随水力停留时间（HRT）的延长而增加。间甲酚进水浓度低于200 mg/L或水力停留时间大于等于3 d时,间甲酚及COD的去除率均可达到90%以上。说明所设计、搭建的藻-膜反应器对间甲酚废水具有较高的去除效率。对反应过后膜丝的污染状况进行了表征,发现反应器内废水及微藻颗粒仅对膜丝表面有轻微污染,对膜孔内部几乎没有影响。
Abstract
jian jia fen zuo wei ben fen de jia ji hua yan sheng wu ,shi fen lei hua ge wu zhong de dai biao xing wu zhi ,yin ju you jiao gao du xing he fu shi xing bei lie wei wo guo you xian kong zhi wu ran wu zhi yi 。jian jia fen bi ben fen ju you geng gao de du xing ,zai jiao di nong du shi bian ke dui ren lei he huan jing zao cheng wei hai 。li yong wei zao qu chu fei shui zhong de fen lei hua ge wu ju you kuai su 、wen ding 、xiao lv gao 、neng hao di deng you dian ,ta ke yi tong guo sheng wu fu ji 、sheng wu jiang jie deng fang shi jiang fei shui zhong de fen lei hua ge wu huo ji ta nan jiang jie you ji wu zhuai hua wei mo du wu zhi ,cong er da dao hua gong gong ye fei shui de pai fang yao qiu 。ben ke ti yi ren gong pei zhi de jian jia fen mo ni fei shui wei yan jiu dui xiang ,yan jiu le jian jia fen sheng wu jiang jie de you shi zao chong ji chu shi zao mi du 、chu shi pei yang ji pHzhi he chu shi jian jia fen nong du dui wei zao sheng wu jiang jie jian jia fen de ying xiang ;bi jiao le dan bai he xiao qiu zao xun hua qian hou dui jian jia fen nai shou xing he qu chu xiao lv de bian hua ;tan jiu le dan bai he xiao qiu zao dui jian jia fen de dai xie tu jing ;bing she ji 、da jian le zao -mo fan ying qi ,kao cha le jian jia fen jin shui nong du he shui li ting liu shi jian dui fan ying qi yun hang de ying xiang 。de chu jie lun ru xia :（1）shua qu dui fen lei wu zhi you jiao hao jiang jie neng li de si chong wei zao :tong lu wei nang zao 、dan bai he xiao qiu zao 、xie sheng shan zao he yu xing zao ,fen bie dui jian jia fen jin hang sheng wu jiang jie ,guan cha si chong wei zao dui jian jia fen de nai shou xing ji jiang jie neng li ,fa xian dan bai he xiao qiu zao dui jian jia fen de jiang jie xiao lv zui jia ;tan tao le bu tong chu shi zao mi du (OD600=0.2、0.4、0.6、0.8、1.0)、bu tong chu shi pHzhi （pH=5.0、6.0、7.0、8.0、9.0）ji bu tong chu shi jian jia fen nong du （100 mg/L、200 mg/L、300 mg/Lhe 400 mg/L）xia dan bai he xiao qiu zao de sheng chang zhuang kuang ji ji dui jian jia fen de qu chu xiao lv ,fa xian dang dan bai he xiao qiu zao de chu shi zao mi du OD600=0.8shi ,jian jia fen de cun zai dui dan bai he xiao qiu zao de sheng chang ju you ming xian de cu jin zuo yong ,ju 4 dnei bian ke jiang 100 mg/Lde jian jia fen wan quan jiang jie ;pei yang ji chu shi pH=7.0shi ,di 6 dzao mi du wei chu shi zao mi du de 1.79bei ,dui jian jia fen de qu chu lv da dao 100%,er suan xing tiao jian xia de jian jia fen qu chu lv jin wei 28.08%;jian jia fen chu shi nong du dui dan bai he xiao qiu zao de sheng chang you hen da de ying xiang ,jian jia fen chu shi nong du yue gao ,dui dan bai he xiao qiu zao de du xing zuo yong yue jiang ,dan bai he xiao qiu zao sheng chang ji dui jian jia fen jiang jie de kuo ying ji yue chang 。tong guo yi ji lie de shi yan ,zui zhong que ding dan bai he xiao qiu zao jiang jie jian jia fen de zui kuo chu shi zao mi du wei OD600=0.8,zui kuo pei yang ji chu shi pH=7.0,zui kuo jian jia fen chu shi nong du wei 100 mg/L。（2）yan jiu le zao chong xun hua dui dan bai he xiao qiu zao de sheng chang ji dui jian jia fen jiang jie neng li de ying xiang 。jie guo biao ming ,xun hua hou dan bai he xiao qiu zao de kuo ying ji su duan ,zao mi du he xie lu su ahan liang ming xian di gao ,300 mg/Lhe 400 mg/Ljian jia fen nong du xie pai xia de zao ti xie lu su ahan liang fen bie shi wei xun hua zao ti de 1.98bei he 3.98bei ;xun hua hou zao ti dui jian jia fen de nai shou xing zeng jia ,jian jia fen de zui da zhi si nong du you 500 mg/Lshang sheng dao le 600mg/L;xun hua hou dan bai he xiao qiu zao zai 35tian nei ke wan quan jiang jie 400 mg/Lde jian jia fen ,biao ming zao chong xun hua ke yi you xiao di gao wei zao de jiang jie neng li 。（3）tan tao le dan bai he xiao qiu zao dui jian jia fen de dai xie tu jing ,li yong FT-IRhe GC-MSde ding xing fen xi gong neng ,jian ce dao jian jia fen dai xie guo cheng zhong you 3-qiang ji ben jia suan he long dan suan deng zhong jian chan wu de sheng cheng ,tui ce jian jia fen ke neng shi tong guo long dan suan tu jing jin hang jiang jie ;jian jia fen shou xian zai ben fen qiang hua mei de zuo yong xia dai xie xing cheng 3-qiang ji ben jia suan ,ran hou jin ru long dan suan tu jing jin yi bu lie jie ,zui zhong xing cheng bing tong suan huo yan hu suo suan jin ru tang jiao jie （Glycolysis）he san suo suan xun huan （TCA）。（4）she ji 、da jian le zao -mo fan ying qi bing dui jian jia fen jin shui nong du ji shui li ting liu shi jian dui zao -mo fan ying qi chu li xiao guo de ying xiang jin hang le yan jiu 。jie guo biao ming ,jian jia fen ji CODde qu chu xiao lv sui jian jia fen jin shui nong du de sheng gao er jiang di ,sui shui li ting liu shi jian （HRT）de yan chang er zeng jia 。jian jia fen jin shui nong du di yu 200 mg/Lhuo shui li ting liu shi jian da yu deng yu 3 dshi ,jian jia fen ji CODde qu chu lv jun ke da dao 90%yi shang 。shui ming suo she ji 、da jian de zao -mo fan ying qi dui jian jia fen fei shui ju you jiao gao de qu chu xiao lv 。dui fan ying guo hou mo si de wu ran zhuang kuang jin hang le biao zheng ,fa xian fan ying qi nei fei shui ji wei zao ke li jin dui mo si biao mian you qing wei wu ran ,dui mo kong nei bu ji hu mei you ying xiang 。
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论文作者分别是来自济南大学的崔娜，发表于刊物济南大学2019-10-31论文，是一篇关于微藻论文,间甲酚论文,生物降解论文,驯化论文,藻膜反应器论文,济南大学2019-10-31论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自济南大学2019-10-31论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。