本文主要研究内容
作者周升妹（2019）在《玉米赤霉烯酮对断奶仔猪肠道相关基因表达的影响》一文中研究指出：玉米赤霉烯酮(ZEA)污染广泛,危害严重,如何有效防控、减少损害是饲料工业乃至畜牧业亟待解决的科学难题。肠道是吸收、代谢和降解ZEA最主要的器官。肠道的黏膜比身体其他部位更多地接触毒素,最易受ZEA及其代谢产物的毒性,因此探讨ZEA对肠道的影响,同时验证专用复合酶的应用效果。本试验旨在研究低剂量ZEA对断奶仔猪肠道屏障防御、氧化应激和养分吸收功能影响。选取30头健康三元杂交(杜×长×大)断奶仔猪(平均体重14.13±0.80 kg),随机分为3个处理,各组间初始体重差异不显著(P>0.05)。对照组饲喂基础饲粮,ZEA1.0组的饲粮是在基础饲粮的基础上添加1 mg/kg ZEA,ZEA1.0+M组饲粮则是在试验1组的基础上添加2 000 mg/kg的专用复合酶。预试期10 d,正试期35 d。结果表明:(1)ZEA1.0组和ZEA1.0+M组的ADG显著高于对照组(P<0.05)。(2)ZEA1.0组显著降低十二指肠和回肠绒毛的绒毛高度和隐窝深度(P<0.01),显著增加空肠的绒毛高度和隐窝深度(P<0.01),显著升高空肠和回肠的绒毛高度/隐窝深度(P<0.05)。(3)与对照组相比,ZEA1.0组十二指肠绒毛损伤,上皮脱落,小肠腺稀疏,十二指肠腺组织变得疏松;空肠绒毛顶端偶见损伤,小肠腺稀疏,形状变短;回肠中集合淋巴结着色较浅,数目增多。与ZEA1.0组相比,ZEA1.0+M组十二指肠绒毛上皮完整,损伤得到修复;空肠中小肠腺密集;回肠淋巴结着色较深。(4)与对照组相比,ZEA1.0组claudin1的mRNA表达量和蛋白表达量在十二指肠显著降低(P<0.01),在回肠显著升高(P<0.01);occludin的mRNA表达量在十二指肠、空肠显著降低(P<0.01),在回肠中显著升高(P<0.01);小肠以及肠系膜淋巴结Nrf2的免疫阳性反应增强,mRNA表达量和蛋白表达量显著升高(P<0.01);CAT1的mRNA表达量和蛋白表达量在空肠、回肠显著降低(P<0.01);SGLT1的mRNA表达量和蛋白表达量显著升高(P<0.05)。以上结果表明,(1)饲粮中添加1 mg/kg的ZEA能够提高断奶仔猪的日增重,降低料重比。(2)饲粮中添加1 mg/kg的ZEA能够影响断奶仔猪小肠屏障功能,十二指肠有破坏作用,对回肠有促进作用。(3)饲粮中添加1 mg/kg的ZEA能够刺激小肠黏膜以及肠系膜淋巴结,产生氧化应激反应。(4)饲粮中添加1 mg/kg的ZEA能促进断奶仔猪小肠对糖的转运,抑制空肠和回肠对氨基酸的转运。(5)专用复合酶能有效缓解ZEA的破坏作用。
Abstract
yu mi chi mei xi tong (ZEA)wu ran an fan ,wei hai yan chong ,ru he you xiao fang kong 、jian shao sun hai shi si liao gong ye nai zhi chu mu ye ji dai jie jue de ke xue nan ti 。chang dao shi xi shou 、dai xie he jiang jie ZEAzui zhu yao de qi guan 。chang dao de nian mo bi shen ti ji ta bu wei geng duo de jie chu du su ,zui yi shou ZEAji ji dai xie chan wu de du xing ,yin ci tan tao ZEAdui chang dao de ying xiang ,tong shi yan zheng zhuan yong fu ge mei de ying yong xiao guo 。ben shi yan zhi zai yan jiu di ji liang ZEAdui duan nai zai zhu chang dao bing zhang fang yu 、yang hua ying ji he yang fen xi shou gong neng ying xiang 。shua qu 30tou jian kang san yuan za jiao (du ×chang ×da )duan nai zai zhu (ping jun ti chong 14.13±0.80 kg),sui ji fen wei 3ge chu li ,ge zu jian chu shi ti chong cha yi bu xian zhe (P>0.05)。dui zhao zu si wei ji chu si liang ,ZEA1.0zu de si liang shi zai ji chu si liang de ji chu shang tian jia 1 mg/kg ZEA,ZEA1.0+Mzu si liang ze shi zai shi yan 1zu de ji chu shang tian jia 2 000 mg/kgde zhuan yong fu ge mei 。yu shi ji 10 d,zheng shi ji 35 d。jie guo biao ming :(1)ZEA1.0zu he ZEA1.0+Mzu de ADGxian zhe gao yu dui zhao zu (P<0.05)。(2)ZEA1.0zu xian zhe jiang di shi er zhi chang he hui chang rong mao de rong mao gao du he yin wo shen du (P<0.01),xian zhe zeng jia kong chang de rong mao gao du he yin wo shen du (P<0.01),xian zhe sheng gao kong chang he hui chang de rong mao gao du /yin wo shen du (P<0.05)。(3)yu dui zhao zu xiang bi ,ZEA1.0zu shi er zhi chang rong mao sun shang ,shang pi tuo la ,xiao chang xian xi shu ,shi er zhi chang xian zu zhi bian de shu song ;kong chang rong mao ding duan ou jian sun shang ,xiao chang xian xi shu ,xing zhuang bian duan ;hui chang zhong ji ge lin ba jie zhao se jiao jian ,shu mu zeng duo 。yu ZEA1.0zu xiang bi ,ZEA1.0+Mzu shi er zhi chang rong mao shang pi wan zheng ,sun shang de dao xiu fu ;kong chang zhong xiao chang xian mi ji ;hui chang lin ba jie zhao se jiao shen 。(4)yu dui zhao zu xiang bi ,ZEA1.0zu claudin1de mRNAbiao da liang he dan bai biao da liang zai shi er zhi chang xian zhe jiang di (P<0.01),zai hui chang xian zhe sheng gao (P<0.01);occludinde mRNAbiao da liang zai shi er zhi chang 、kong chang xian zhe jiang di (P<0.01),zai hui chang zhong xian zhe sheng gao (P<0.01);xiao chang yi ji chang ji mo lin ba jie Nrf2de mian yi yang xing fan ying zeng jiang ,mRNAbiao da liang he dan bai biao da liang xian zhe sheng gao (P<0.01);CAT1de mRNAbiao da liang he dan bai biao da liang zai kong chang 、hui chang xian zhe jiang di (P<0.01);SGLT1de mRNAbiao da liang he dan bai biao da liang xian zhe sheng gao (P<0.05)。yi shang jie guo biao ming ,(1)si liang zhong tian jia 1 mg/kgde ZEAneng gou di gao duan nai zai zhu de ri zeng chong ,jiang di liao chong bi 。(2)si liang zhong tian jia 1 mg/kgde ZEAneng gou ying xiang duan nai zai zhu xiao chang bing zhang gong neng ,shi er zhi chang you po huai zuo yong ,dui hui chang you cu jin zuo yong 。(3)si liang zhong tian jia 1 mg/kgde ZEAneng gou ci ji xiao chang nian mo yi ji chang ji mo lin ba jie ,chan sheng yang hua ying ji fan ying 。(4)si liang zhong tian jia 1 mg/kgde ZEAneng cu jin duan nai zai zhu xiao chang dui tang de zhuai yun ,yi zhi kong chang he hui chang dui an ji suan de zhuai yun 。(5)zhuan yong fu ge mei neng you xiao huan jie ZEAde po huai zuo yong 。
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论文详细介绍
论文作者分别是来自山东农业大学的周升妹，发表于刊物山东农业大学2019-07-08论文，是一篇关于断奶仔猪论文,玉米赤霉烯酮论文,小肠论文,紧密连接蛋白论文,氧化应激论文,山东农业大学2019-07-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自山东农业大学2019-07-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：断奶仔猪论文; 玉米赤霉烯酮论文; 小肠论文; 紧密连接蛋白论文; 氧化应激论文; 山东农业大学2019-07-08论文;