本文主要研究内容
作者黄艳（2019）在《Orexin-A对脊髓腹角神经元基本电生理特性及N型乙酰胆碱受体的作用》一文中研究指出：目的:研究orexin-A对脊髓腹角神经元基本电生理参数及烟碱型乙酰胆碱受体(n ACh R)的作用。方法:使用7-12 d的新生SD大鼠。乙醚辅助冰浴麻醉后,将含有腰骶膨大的脊髓游离,制备4-5片厚度为350-500μm的脊髓切片,孵育约0.5 h后,用木瓜蛋白酶(papain,0.18 g/30 ml人工脑脊液)消化切片20-25 min,终止消化并孵育50 min。显微镜下选取腹角,使用抛光的巴斯德吸管进行神经元的急性机械分离。对贴壁的健康神经元进行穿孔膜片钳记录。结果:(1)急性分离的脊髓腹角神经元状态良好,具有形状多样的胞体且立体饱满,以及完整的突起并发出多条分支。(2)9个急性分离的脊髓腹角神经元有自发动作电位,其中5个神经元的放电频率为(5.88±1.87)Hz,4个神经元的放电频率为(16.75±5.40)Hz,两者有显著差异(P<0.01)。低频放电的神经元静息电位为(-66.54±6.48)m V、动作电位幅度为(76.08±10.07)m V,高频放电的神经元静息电位为(-52.74±5.27)m V、动作电位幅度为(55.54±7.46)m V,两者均有显著差异(P<0.05)。细胞其余电生理参数无明显差异。(3)持续灌流orexin-A(OXA)2 min后,在5例细胞中记录到4个脊髓腹角神经元的自发动作电位放电频率较灌流前增加了(63.64±5.25)%,幅度等参数无明显变化;(4)在15个神经元中,有12例细胞给予0.3 mmol/L尼古丁诱导出内向电流,幅度为(142.97±75.02)p A,用100 nmol/L OXA进行2 min的预处理,可显著抑制电流幅度至(69.07±61.07)p A(P<0.001),抑制率为(54.75±22.62)%。(5)在9个神经元中,有7例细胞给予尼古丁诱导的电流幅度为(127.94±68.78)p A,将100 nmol/L OXA共同施用于神经元,尼古丁诱导的电流幅度降为(64.58±34.03)p A,在此基础上,通过施用orexin-1受体(OX1R)选择性拮抗剂SB334867(10μmol/L)2min后可阻断OXA对尼古丁诱导电流的抑制作用,电流幅值为(111.06±58.25)p A。因此,SB334867可取消OXA对尼古丁电流的抑制作用。但对于另两例神经元,SB334867并未阻断OXA的抑制作用。结论:实验所分离出的脊髓腹角神经元胞体较大而立体,突起多且多分支,细胞状态良好;记录的神经元自发动作电位,根据放电频率可分为低频、高频两类,且这两类细胞的静息电位与动作电位幅度有明显差异,提示可能在此实验中所钳制的细胞有两种及以上;orexin-A可增加脊髓腹角神经元的放电频率,但其他电生理参数无明显变化;orexin-A可能通过OX1R对脊髓大部分腹角神经元的n ACh R具有抑制作用,但不能排除orexin-2受体(OX2R)也参与OXA作用的可能性。
Abstract
mu de :yan jiu orexin-Adui ji sui fu jiao shen jing yuan ji ben dian sheng li can shu ji yan jian xing yi xian dan jian shou ti (n ACh R)de zuo yong 。fang fa :shi yong 7-12 dde xin sheng SDda shu 。yi mi fu zhu bing yu ma zui hou ,jiang han you yao di peng da de ji sui you li ,zhi bei 4-5pian hou du wei 350-500μmde ji sui qie pian ,fu yo yao 0.5 hhou ,yong mu gua dan bai mei (papain,0.18 g/30 mlren gong nao ji ye )xiao hua qie pian 20-25 min,zhong zhi xiao hua bing fu yo 50 min。xian wei jing xia shua qu fu jiao ,shi yong pao guang de ba si de xi guan jin hang shen jing yuan de ji xing ji xie fen li 。dui tie bi de jian kang shen jing yuan jin hang chuan kong mo pian qian ji lu 。jie guo :(1)ji xing fen li de ji sui fu jiao shen jing yuan zhuang tai liang hao ,ju you xing zhuang duo yang de bao ti ju li ti bao man ,yi ji wan zheng de tu qi bing fa chu duo tiao fen zhi 。(2)9ge ji xing fen li de ji sui fu jiao shen jing yuan you zi fa dong zuo dian wei ,ji zhong 5ge shen jing yuan de fang dian pin lv wei (5.88±1.87)Hz,4ge shen jing yuan de fang dian pin lv wei (16.75±5.40)Hz,liang zhe you xian zhe cha yi (P<0.01)。di pin fang dian de shen jing yuan jing xi dian wei wei (-66.54±6.48)m V、dong zuo dian wei fu du wei (76.08±10.07)m V,gao pin fang dian de shen jing yuan jing xi dian wei wei (-52.74±5.27)m V、dong zuo dian wei fu du wei (55.54±7.46)m V,liang zhe jun you xian zhe cha yi (P<0.05)。xi bao ji yu dian sheng li can shu mo ming xian cha yi 。(3)chi xu guan liu orexin-A(OXA)2 minhou ,zai 5li xi bao zhong ji lu dao 4ge ji sui fu jiao shen jing yuan de zi fa dong zuo dian wei fang dian pin lv jiao guan liu qian zeng jia le (63.64±5.25)%,fu du deng can shu mo ming xian bian hua ;(4)zai 15ge shen jing yuan zhong ,you 12li xi bao gei yu 0.3 mmol/Lni gu ding you dao chu nei xiang dian liu ,fu du wei (142.97±75.02)p A,yong 100 nmol/L OXAjin hang 2 minde yu chu li ,ke xian zhe yi zhi dian liu fu du zhi (69.07±61.07)p A(P<0.001),yi zhi lv wei (54.75±22.62)%。(5)zai 9ge shen jing yuan zhong ,you 7li xi bao gei yu ni gu ding you dao de dian liu fu du wei (127.94±68.78)p A,jiang 100 nmol/L OXAgong tong shi yong yu shen jing yuan ,ni gu ding you dao de dian liu fu du jiang wei (64.58±34.03)p A,zai ci ji chu shang ,tong guo shi yong orexin-1shou ti (OX1R)shua ze xing jie kang ji SB334867(10μmol/L)2minhou ke zu duan OXAdui ni gu ding you dao dian liu de yi zhi zuo yong ,dian liu fu zhi wei (111.06±58.25)p A。yin ci ,SB334867ke qu xiao OXAdui ni gu ding dian liu de yi zhi zuo yong 。dan dui yu ling liang li shen jing yuan ,SB334867bing wei zu duan OXAde yi zhi zuo yong 。jie lun :shi yan suo fen li chu de ji sui fu jiao shen jing yuan bao ti jiao da er li ti ,tu qi duo ju duo fen zhi ,xi bao zhuang tai liang hao ;ji lu de shen jing yuan zi fa dong zuo dian wei ,gen ju fang dian pin lv ke fen wei di pin 、gao pin liang lei ,ju zhe liang lei xi bao de jing xi dian wei yu dong zuo dian wei fu du you ming xian cha yi ,di shi ke neng zai ci shi yan zhong suo qian zhi de xi bao you liang chong ji yi shang ;orexin-Ake zeng jia ji sui fu jiao shen jing yuan de fang dian pin lv ,dan ji ta dian sheng li can shu mo ming xian bian hua ;orexin-Ake neng tong guo OX1Rdui ji sui da bu fen fu jiao shen jing yuan de n ACh Rju you yi zhi zuo yong ,dan bu neng pai chu orexin-2shou ti (OX2R)ye can yu OXAzuo yong de ke neng xing 。
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论文详细介绍
论文作者分别是来自皖南医学院的黄艳，发表于刊物皖南医学院2019-09-06论文，是一篇关于脊髓腹角神经元论文,型乙酰胆碱受体论文,穿孔膜片钳记录论文,皖南医学院2019-09-06论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自皖南医学院2019-09-06论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。