本文主要研究内容
作者周爽（2019）在《基于萘酰亚胺-吲哚荧光母体活性硫探针的设计合成及其性质研究》一文中研究指出：荧光探针具有高的灵敏度和优良的选择性,被广泛应用于荧光传感和医疗诊断等生命科学研究领域,因此开发出性能优越的新型荧光探针具有深远的意义。由萘酰亚胺和吲哚杂化的荧光染料TPFC4具有较长的发射波长(红色荧光)和较大的Stokes位移(大于100 nm),是构建荧光探针优选的母体结构。本文以染料TPFC4为母体,设计合成了三种活性硫荧光探针,对苯硫酚特异性识别的荧光探针TPFC-DNB,能区分检测巯基氨基酸与非巯基氨基酸的荧光探针TPFC-DNBS,可以区分GSH与Cys/Hcy的荧光探针TPFC-SePh。具体内容如下:(1)以2,4-二硝基苯基(DNB)为苯硫酚的识别基团,与荧光染料TPFC4通过醚键连接构建出探针TPFC-DNB。由于识别基团与荧光母体间的光诱导电子转移(PET)过程,使探针的荧光淬灭。当苯硫酚与探针反应时,发生亲核取代反应,识别基团离去,PET过程受到抑制,荧光得到恢复。探针TPFC-DNB在应用过程中表现出了大Stokes位移(140 nm),红光发射(590 nm),优异的选择性和较低的检出限(36 nM)以及快速的响应,并且成功应用于活细胞中苯硫酚的检测。(2)以2,4-二硝基苯磺酰基(DNBS)为识别基团构建了荧光探针TPFC-DNBS,由于识别基团与染料TPFC4之间的PET过程而使探针无荧光。在与巯基氨基酸(Cys、Hcy和GSH)的作用下,磺酰酯键断裂,释放出染料TPFC4,发射出强烈荧光信号。探针TPFC-DNBS的发射波长在590 nm,Stokes位移为137nm,并且能成功检测活细胞内源性和外源性的巯基氨基酸。(3)以苯硒醚为识别基团,结合香豆素与TPFC4构建了荧光探针TPFC-SePh,该探针能够将GSH与Cys、Hcy及其他氨基酸区分开。GSH与识别基团发生亲核取代反应,苯硒基的部分离去,产生强烈荧光信号;Cys/Hcy与探针反应,识别基团离去后产物继续发生分子内重排反应,致使发射出弱的荧光信号。该探针检测过程中的发射波长为570 nm,Stokes位移为120 nm,表现出高选择性和强抗干扰性。
Abstract
ying guang tan zhen ju you gao de ling min du he you liang de shua ze xing ,bei an fan ying yong yu ying guang chuan gan he yi liao zhen duan deng sheng ming ke xue yan jiu ling yu ,yin ci kai fa chu xing neng you yue de xin xing ying guang tan zhen ju you shen yuan de yi yi 。you nai xian ya an he yin duo za hua de ying guang ran liao TPFC4ju you jiao chang de fa she bo chang (gong se ying guang )he jiao da de Stokeswei yi (da yu 100 nm),shi gou jian ying guang tan zhen you shua de mu ti jie gou 。ben wen yi ran liao TPFC4wei mu ti ,she ji ge cheng le san chong huo xing liu ying guang tan zhen ,dui ben liu fen te yi xing shi bie de ying guang tan zhen TPFC-DNB,neng ou fen jian ce qiu ji an ji suan yu fei qiu ji an ji suan de ying guang tan zhen TPFC-DNBS,ke yi ou fen GSHyu Cys/Hcyde ying guang tan zhen TPFC-SePh。ju ti nei rong ru xia :(1)yi 2,4-er xiao ji ben ji (DNB)wei ben liu fen de shi bie ji tuan ,yu ying guang ran liao TPFC4tong guo mi jian lian jie gou jian chu tan zhen TPFC-DNB。you yu shi bie ji tuan yu ying guang mu ti jian de guang you dao dian zi zhuai yi (PET)guo cheng ,shi tan zhen de ying guang cui mie 。dang ben liu fen yu tan zhen fan ying shi ,fa sheng qin he qu dai fan ying ,shi bie ji tuan li qu ,PETguo cheng shou dao yi zhi ,ying guang de dao hui fu 。tan zhen TPFC-DNBzai ying yong guo cheng zhong biao xian chu le da Stokeswei yi (140 nm),gong guang fa she (590 nm),you yi de shua ze xing he jiao di de jian chu xian (36 nM)yi ji kuai su de xiang ying ,bing ju cheng gong ying yong yu huo xi bao zhong ben liu fen de jian ce 。(2)yi 2,4-er xiao ji ben huang xian ji (DNBS)wei shi bie ji tuan gou jian le ying guang tan zhen TPFC-DNBS,you yu shi bie ji tuan yu ran liao TPFC4zhi jian de PETguo cheng er shi tan zhen mo ying guang 。zai yu qiu ji an ji suan (Cys、Hcyhe GSH)de zuo yong xia ,huang xian zhi jian duan lie ,shi fang chu ran liao TPFC4,fa she chu jiang lie ying guang xin hao 。tan zhen TPFC-DNBSde fa she bo chang zai 590 nm,Stokeswei yi wei 137nm,bing ju neng cheng gong jian ce huo xi bao nei yuan xing he wai yuan xing de qiu ji an ji suan 。(3)yi ben xi mi wei shi bie ji tuan ,jie ge xiang dou su yu TPFC4gou jian le ying guang tan zhen TPFC-SePh,gai tan zhen neng gou jiang GSHyu Cys、Hcyji ji ta an ji suan ou fen kai 。GSHyu shi bie ji tuan fa sheng qin he qu dai fan ying ,ben xi ji de bu fen li qu ,chan sheng jiang lie ying guang xin hao ;Cys/Hcyyu tan zhen fan ying ,shi bie ji tuan li qu hou chan wu ji xu fa sheng fen zi nei chong pai fan ying ,zhi shi fa she chu ruo de ying guang xin hao 。gai tan zhen jian ce guo cheng zhong de fa she bo chang wei 570 nm,Stokeswei yi wei 120 nm,biao xian chu gao shua ze xing he jiang kang gan rao xing 。
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论文详细介绍
论文作者分别是来自郑州大学的周爽，发表于刊物郑州大学2019-07-09论文，是一篇关于荧光探针论文,萘酰亚胺吲哚类染料论文,苯硫酚论文,巯基氨基酸论文,谷胱甘肽论文,郑州大学2019-07-09论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自郑州大学2019-07-09论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：荧光探针论文; 萘酰亚胺吲哚类染料论文; 苯硫酚论文; 巯基氨基酸论文; 谷胱甘肽论文; 郑州大学2019-07-09论文;