本文主要研究内容
作者张莹佳（2019）在《含精氨酸的聚酯脲氨酯复合埃洛石材料的骨修复研究》一文中研究指出：多嵌段结构的热塑性医用聚氨酯弹性体(Thermoplastic polyurethane,TPU)力学性能优良,成型加工方式多,在人工血管、导管、心脏补片、骨和软骨替代中有广泛应用。但TPU材料仍存在亲水性、生物活性、降解性和细胞相容性有待提高的问题,距骨组织修复与再生材料的需求仍有差距。本研究针对聚酯型聚氨酯疏水性强而导致的降解速率慢、细胞粘附性以及成骨活性不佳的问题,尝试将精氨酸引入聚氨酯结构以改善其亲水性,同时将合成聚氨酯与埃洛石复合以改善其成骨特性。研究首先将苯磺酸保护的精氨酸与二醇(碳原子数x=2,4,6,8)进行缩合反应得到氨端基精氨酸二酯(L-Arg-x),红外、核磁等分析测试结果表明得到与设计相一致的L-Arg-x分子,实验结果表明随着碳原子数的增加,反应收率逐渐提高,从17.63%到67.30%。采用收率最高的两种精氨酸二醇酯合成了一系列具有精氨酸含量的聚酯脲氨酯。测试结果表明精氨酸的引入显著改善了亲水性,随着精氨酸的引入,接触角从109.67±3.92~o降低到59.10±0.61~o。膜表面Zeta电位(pH~7)从-20 mV提高到130 mV。材料降解性能也得到显著改善,在60天时,不含精氨酸的聚氨酯材料失重率不足1%,不同精氨酸含量的材料的失重率提升到6%~30%。聚酯脲氨酯的生物学评价显示合成材料无细胞毒性,细胞在材料表面的粘附与增殖显著改善,但精氨酸含量偏高(A8-2.0材料)也会导致粘附活细胞比例减少。细胞迁移实验还表明,材料表面的正电荷利于干细胞迁移。骨髓基质干细胞在精氨酸聚酯脲氨酯材料表面的成骨诱导研究表明,干细胞的COL1和BMP4表达水平上调。埃洛石纳米管与聚酯脲氨酯复合提高了材料的力学性能,电镜观察表明埃洛石在材料内部分散均匀。细胞体外矿化结果显示埃洛石纳米管(Halloysite nanotubes,HNTs)可有效增强牙髓干细胞(Human dental pulp stem cells,hDPSC)体外矿化作用,A8-1.2/6%HNTs的复合材料效果最为明显,同时,细胞成骨实验还表明,在含6%HNTs的复合材料上,COL1、BMP4和RUNX2的表达水平显著上调,hDPSC细胞成骨分化能力提高。大鼠颅骨缺损模型修复实验表明,A8-1.2/6%HNTs复合材料补片可显著促进临界尺寸颅骨缺损的修复,在12周时,缺损处相对骨体积分数(BV/TV)达到(71.65±11.40)%,显著优于未加HNTs的材料组。巨噬细胞在PEUU和PEUU/HNTs上的共培养研究表明,在聚酯脲氨酯及其埃洛石复合材料上,巨噬细胞分泌促炎因子TNF-α水平下调,破骨相关因子IL-6和OSM的分泌上调,此外,成骨相关因子BMP2在A8-1.2和A8-1.2/6%HNTs上都显著上调,A8-1.2和A8-1.2/6%HNTs材料上早期巨噬细胞调节的破骨活动和促骨生成可能对骨重塑起到了重要作用,从而使A8-1.2/6%HNTs具有更好的骨修复效果。研究合成出来氨端基精氨酸二醇酯并以此为基础合成除了含精氨酸结构的可降解聚酯脲氨酯材料,改善了合成聚氨酯亲水性、降解性和细胞相容性,并制备出了该聚氨酯与HNTs的复合材料,复合材料具有促进骨再生的能力。
Abstract
duo qian duan jie gou de re su xing yi yong ju an zhi dan xing ti (Thermoplastic polyurethane,TPU)li xue xing neng you liang ,cheng xing jia gong fang shi duo ,zai ren gong xie guan 、dao guan 、xin zang bu pian 、gu he ruan gu ti dai zhong you an fan ying yong 。dan TPUcai liao reng cun zai qin shui xing 、sheng wu huo xing 、jiang jie xing he xi bao xiang rong xing you dai di gao de wen ti ,ju gu zu zhi xiu fu yu zai sheng cai liao de xu qiu reng you cha ju 。ben yan jiu zhen dui ju zhi xing ju an zhi shu shui xing jiang er dao zhi de jiang jie su lv man 、xi bao nian fu xing yi ji cheng gu huo xing bu jia de wen ti ,chang shi jiang jing an suan yin ru ju an zhi jie gou yi gai shan ji qin shui xing ,tong shi jiang ge cheng ju an zhi yu ai luo dan fu ge yi gai shan ji cheng gu te xing 。yan jiu shou xian jiang ben huang suan bao hu de jing an suan yu er chun (tan yuan zi shu x=2,4,6,8)jin hang su ge fan ying de dao an duan ji jing an suan er zhi (L-Arg-x),gong wai 、he ci deng fen xi ce shi jie guo biao ming de dao yu she ji xiang yi zhi de L-Arg-xfen zi ,shi yan jie guo biao ming sui zhao tan yuan zi shu de zeng jia ,fan ying shou lv zhu jian di gao ,cong 17.63%dao 67.30%。cai yong shou lv zui gao de liang chong jing an suan er chun zhi ge cheng le yi ji lie ju you jing an suan han liang de ju zhi niao an zhi 。ce shi jie guo biao ming jing an suan de yin ru xian zhe gai shan le qin shui xing ,sui zhao jing an suan de yin ru ,jie chu jiao cong 109.67±3.92~ojiang di dao 59.10±0.61~o。mo biao mian Zetadian wei (pH~7)cong -20 mVdi gao dao 130 mV。cai liao jiang jie xing neng ye de dao xian zhe gai shan ,zai 60tian shi ,bu han jing an suan de ju an zhi cai liao shi chong lv bu zu 1%,bu tong jing an suan han liang de cai liao de shi chong lv di sheng dao 6%~30%。ju zhi niao an zhi de sheng wu xue ping jia xian shi ge cheng cai liao mo xi bao du xing ,xi bao zai cai liao biao mian de nian fu yu zeng shi xian zhe gai shan ,dan jing an suan han liang pian gao (A8-2.0cai liao )ye hui dao zhi nian fu huo xi bao bi li jian shao 。xi bao qian yi shi yan hai biao ming ,cai liao biao mian de zheng dian he li yu gan xi bao qian yi 。gu sui ji zhi gan xi bao zai jing an suan ju zhi niao an zhi cai liao biao mian de cheng gu you dao yan jiu biao ming ,gan xi bao de COL1he BMP4biao da shui ping shang diao 。ai luo dan na mi guan yu ju zhi niao an zhi fu ge di gao le cai liao de li xue xing neng ,dian jing guan cha biao ming ai luo dan zai cai liao nei bu fen san jun yun 。xi bao ti wai kuang hua jie guo xian shi ai luo dan na mi guan (Halloysite nanotubes,HNTs)ke you xiao zeng jiang ya sui gan xi bao (Human dental pulp stem cells,hDPSC)ti wai kuang hua zuo yong ,A8-1.2/6%HNTsde fu ge cai liao xiao guo zui wei ming xian ,tong shi ,xi bao cheng gu shi yan hai biao ming ,zai han 6%HNTsde fu ge cai liao shang ,COL1、BMP4he RUNX2de biao da shui ping xian zhe shang diao ,hDPSCxi bao cheng gu fen hua neng li di gao 。da shu lu gu que sun mo xing xiu fu shi yan biao ming ,A8-1.2/6%HNTsfu ge cai liao bu pian ke xian zhe cu jin lin jie che cun lu gu que sun de xiu fu ,zai 12zhou shi ,que sun chu xiang dui gu ti ji fen shu (BV/TV)da dao (71.65±11.40)%,xian zhe you yu wei jia HNTsde cai liao zu 。ju shi xi bao zai PEUUhe PEUU/HNTsshang de gong pei yang yan jiu biao ming ,zai ju zhi niao an zhi ji ji ai luo dan fu ge cai liao shang ,ju shi xi bao fen bi cu yan yin zi TNF-αshui ping xia diao ,po gu xiang guan yin zi IL-6he OSMde fen bi shang diao ,ci wai ,cheng gu xiang guan yin zi BMP2zai A8-1.2he A8-1.2/6%HNTsshang dou xian zhe shang diao ,A8-1.2he A8-1.2/6%HNTscai liao shang zao ji ju shi xi bao diao jie de po gu huo dong he cu gu sheng cheng ke neng dui gu chong su qi dao le chong yao zuo yong ,cong er shi A8-1.2/6%HNTsju you geng hao de gu xiu fu xiao guo 。yan jiu ge cheng chu lai an duan ji jing an suan er chun zhi bing yi ci wei ji chu ge cheng chu le han jing an suan jie gou de ke jiang jie ju zhi niao an zhi cai liao ,gai shan le ge cheng ju an zhi qin shui xing 、jiang jie xing he xi bao xiang rong xing ,bing zhi bei chu le gai ju an zhi yu HNTsde fu ge cai liao ,fu ge cai liao ju you cu jin gu zai sheng de neng li 。
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论文作者分别是来自华南理工大学的张莹佳，发表于刊物华南理工大学2019-10-23论文，是一篇关于精氨酸论文,聚酯脲氨酯论文,埃洛石纳米管论文,骨修复论文,巨噬细胞论文,华南理工大学2019-10-23论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自华南理工大学2019-10-23论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：精氨酸论文; 聚酯脲氨酯论文; 埃洛石纳米管论文; 骨修复论文; 巨噬细胞论文; 华南理工大学2019-10-23论文;