论文摘要
目的:木芙蓉属槿葵科木属植物,其主要成分为黄酮甙,包括槲皮甙（uercitrin）、异槲皮甙（isoqercitrin）、金丝桃甙（hyperoside）和绣线菊甙（piracoside）,及花色甙等,为测定木芙蓉主要成分,本实验建立木芙蓉中总黄酮的含量测定方法。方法:波长的选择,取标准溶液及样品提取液在RF-540检测器上进行扫描,发现在300～400nm间有最大吸收值,而本次实验选用370nm为检测波长。测定木芙蓉中黄酮的方法很多,而应用高效液相法检测则是第一次。色谱条件色谱条件:色谱柱C18;流动相:含1%冰醋酸的磷酸二氢钾-甲醇（73∶27,V∶V）;流速:1.0 ml/min;柱温:25℃检测器:SPD-2AS UV检测器;进样体积20μl,手动进样,灵敏度:AUFS=0.8;在上述色谱条件下,对照品溶液和样品溶液分别进样分析。根据高效液相法得到木芙蓉和芦丁色谱图结果;然后计算出木芙蓉中所含总黄酮的含量。结果:应用反向高效液相法测定木芙蓉中总黄酮含量,其样品及标准品基线分离良好,保留时间适中,从色谱效果看非常理想。其回归方程为:Y=0.01X+12.08,本方法简单,且能快速、简便、准确地测定木芙蓉中的总黄酮的含量测定,而且在6小时内稳定,RSD=1.73%;精密度试验（n=6）RSD均在5%以下;芦丁的回收率99.88%, RSD=1.54%。结论:黄酮类化合物是植物中一类多酚化合物,具有多种药理作
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