本文主要研究内容
作者梁飞阳（2019）在《马铃薯疮痂病病原菌AMCC400023的系统鉴定及其致病岛的基因组学分析》一文中研究指出：马铃薯是仅次于小麦、水稻和玉米的世界第四大粮食作物,因营养丰富、膳食纤维高而被誉为“地球未来的粮食”,在全球范围内有着广泛种植。我国是世界马铃薯生产和消费第一大国,并于2015年启动了马铃薯主粮化国家战略。马铃薯疮痂病(potato common scab,PCS)是由植物致病性链霉菌(Streptomyces spp.)引起的土传性病害,在全世界马铃薯产区均有发生,且有逐年加重的趋势。本研究首先对福建、广东和西藏等地区患有疮痂病的马铃薯薯块进行病原菌的分离筛选,用致病基因txtAB,tomA和nec1作PCR扩增,初步筛选出具有致病基因的放线菌菌株,通过形态学和分子生物学进行马铃薯疮痂病病原菌的鉴定,并通过萝卜幼苗法、薯片法和盆栽回接试验验证所筛选病原菌株的致病性。在此基础上,对本研究室分离保藏的马铃薯疮痂病病原菌Streptomyces sp.AMCC400023菌株进行了严格的致病性验证及形态学、生理生化和16S rRNA分子鉴定,并进一步开展了该病原菌的全基因组测序,通过ANI和isDDH分析、全基因组系统发育分析,从基因组水平上最终将Streptomyces sp.AMCC400023鉴定为与普通疮痂病原菌Streptomyces scabiei亲缘关系较近的另类未定名分类种。同时,对Streptomyces sp.AMCC400023菌株的致病岛PAI组分进行了基因组学分析,构建了该病原菌株的PAI组成图,通过ThxA毒素合成基因和核心基因组交联树以及相关马铃薯疮痂病病原菌共线性分析,推测Streptomyces sp.AMCC400023的致病岛PAI是从最原始的S.scabiei中通过基因水平转移(HGT)而来。研究获得的主要结果如下:1.对福建、广东和西藏三个地区的患病马铃薯薯块进行疮痂病病原菌的分离筛选,共分离得到138株具有放线菌特征的分离物。2.对分离获得的放线菌分离物进行致病性验证,同时对具有致病性的菌株进行形态学和16S rRNA鉴定,鉴定结果均是疮痂病病原菌Streptomyce turgidiscabies。3.对本研究室分离保藏的Streptomyces sp.AMCC400023菌株进行了系统的致病性验证,包括萝卜幼苗法、小薯片法、盆栽回接试验和毒素产生试验,所有试验结果均表明Streptomyces sp.AMCC400023具有疮痂病致病性,能够形成典型的疮痂症状。4.在形态学、生理生化和16S rRNA分子鉴定基础上,进一步对Streptomyces sp.AMCC400023菌株进行了全基因组测序,通过ANI和isDDH分析、全基因组系统发育分析,最终将Streptomyces sp.AMCC400023鉴定为与普通疮痂病原菌Streptomyces scabiei亲缘关系较近的另类未定名分类种。5.利用Streptomyces sp.AMCC400023全基因组测序结果,构建了该菌株PAI组成图,证明该菌株的PAI中含有txtAB、nec1和tomA基因以及完整的TR区和CR区。同时thaxtominA合成试验,也证实Streptomyces sp.AMCC400023菌株能够产生thaxtomin A毒素。6.在基因组学基础上,通过对31株致病和非致病链霉菌系统发育分析、ThxA毒素合成基因和核心基因组交联树分析以及相关马铃薯疮痂病病原菌共线性分析,发现Streptomyces sp.AMCC400023与普通疮痂病原菌模式种Streptomyces scabiei NRRL B-16523存在相对较近的亲缘关系,Streptomyces sp.AMCC400023与Streptomyces scabiei87-22在共线性图上存在基因水平转移证据,因此推测Streptomyces sp.AMCC400023的致病岛PAI是从最原始的马铃薯疮痂病病原菌S.scabiei通过基因水平转移(HGT)而来。
Abstract
ma ling shu shi jin ci yu xiao mai 、shui dao he yu mi de shi jie di si da liang shi zuo wu ,yin ying yang feng fu 、shan shi qian wei gao er bei yu wei “de qiu wei lai de liang shi ”,zai quan qiu fan wei nei you zhao an fan chong zhi 。wo guo shi shi jie ma ling shu sheng chan he xiao fei di yi da guo ,bing yu 2015nian qi dong le ma ling shu zhu liang hua guo jia zhan lve 。ma ling shu chuang jia bing (potato common scab,PCS)shi you zhi wu zhi bing xing lian mei jun (Streptomyces spp.)yin qi de tu chuan xing bing hai ,zai quan shi jie ma ling shu chan ou jun you fa sheng ,ju you zhu nian jia chong de qu shi 。ben yan jiu shou xian dui fu jian 、an dong he xi cang deng de ou huan you chuang jia bing de ma ling shu shu kuai jin hang bing yuan jun de fen li shai shua ,yong zhi bing ji yin txtAB,tomAhe nec1zuo PCRkuo zeng ,chu bu shai shua chu ju you zhi bing ji yin de fang xian jun jun zhu ,tong guo xing tai xue he fen zi sheng wu xue jin hang ma ling shu chuang jia bing bing yuan jun de jian ding ,bing tong guo luo bo you miao fa 、shu pian fa he pen zai hui jie shi yan yan zheng suo shai shua bing yuan jun zhu de zhi bing xing 。zai ci ji chu shang ,dui ben yan jiu shi fen li bao cang de ma ling shu chuang jia bing bing yuan jun Streptomyces sp.AMCC400023jun zhu jin hang le yan ge de zhi bing xing yan zheng ji xing tai xue 、sheng li sheng hua he 16S rRNAfen zi jian ding ,bing jin yi bu kai zhan le gai bing yuan jun de quan ji yin zu ce xu ,tong guo ANIhe isDDHfen xi 、quan ji yin zu ji tong fa yo fen xi ,cong ji yin zu shui ping shang zui zhong jiang Streptomyces sp.AMCC400023jian ding wei yu pu tong chuang jia bing yuan jun Streptomyces scabieiqin yuan guan ji jiao jin de ling lei wei ding ming fen lei chong 。tong shi ,dui Streptomyces sp.AMCC400023jun zhu de zhi bing dao PAIzu fen jin hang le ji yin zu xue fen xi ,gou jian le gai bing yuan jun zhu de PAIzu cheng tu ,tong guo ThxAdu su ge cheng ji yin he he xin ji yin zu jiao lian shu yi ji xiang guan ma ling shu chuang jia bing bing yuan jun gong xian xing fen xi ,tui ce Streptomyces sp.AMCC400023de zhi bing dao PAIshi cong zui yuan shi de S.scabieizhong tong guo ji yin shui ping zhuai yi (HGT)er lai 。yan jiu huo de de zhu yao jie guo ru xia :1.dui fu jian 、an dong he xi cang san ge de ou de huan bing ma ling shu shu kuai jin hang chuang jia bing bing yuan jun de fen li shai shua ,gong fen li de dao 138zhu ju you fang xian jun te zheng de fen li wu 。2.dui fen li huo de de fang xian jun fen li wu jin hang zhi bing xing yan zheng ,tong shi dui ju you zhi bing xing de jun zhu jin hang xing tai xue he 16S rRNAjian ding ,jian ding jie guo jun shi chuang jia bing bing yuan jun Streptomyce turgidiscabies。3.dui ben yan jiu shi fen li bao cang de Streptomyces sp.AMCC400023jun zhu jin hang le ji tong de zhi bing xing yan zheng ,bao gua luo bo you miao fa 、xiao shu pian fa 、pen zai hui jie shi yan he du su chan sheng shi yan ,suo you shi yan jie guo jun biao ming Streptomyces sp.AMCC400023ju you chuang jia bing zhi bing xing ,neng gou xing cheng dian xing de chuang jia zheng zhuang 。4.zai xing tai xue 、sheng li sheng hua he 16S rRNAfen zi jian ding ji chu shang ,jin yi bu dui Streptomyces sp.AMCC400023jun zhu jin hang le quan ji yin zu ce xu ,tong guo ANIhe isDDHfen xi 、quan ji yin zu ji tong fa yo fen xi ,zui zhong jiang Streptomyces sp.AMCC400023jian ding wei yu pu tong chuang jia bing yuan jun Streptomyces scabieiqin yuan guan ji jiao jin de ling lei wei ding ming fen lei chong 。5.li yong Streptomyces sp.AMCC400023quan ji yin zu ce xu jie guo ,gou jian le gai jun zhu PAIzu cheng tu ,zheng ming gai jun zhu de PAIzhong han you txtAB、nec1he tomAji yin yi ji wan zheng de TRou he CRou 。tong shi thaxtominAge cheng shi yan ,ye zheng shi Streptomyces sp.AMCC400023jun zhu neng gou chan sheng thaxtomin Adu su 。6.zai ji yin zu xue ji chu shang ,tong guo dui 31zhu zhi bing he fei zhi bing lian mei jun ji tong fa yo fen xi 、ThxAdu su ge cheng ji yin he he xin ji yin zu jiao lian shu fen xi yi ji xiang guan ma ling shu chuang jia bing bing yuan jun gong xian xing fen xi ,fa xian Streptomyces sp.AMCC400023yu pu tong chuang jia bing yuan jun mo shi chong Streptomyces scabiei NRRL B-16523cun zai xiang dui jiao jin de qin yuan guan ji ,Streptomyces sp.AMCC400023yu Streptomyces scabiei87-22zai gong xian xing tu shang cun zai ji yin shui ping zhuai yi zheng ju ,yin ci tui ce Streptomyces sp.AMCC400023de zhi bing dao PAIshi cong zui yuan shi de ma ling shu chuang jia bing bing yuan jun S.scabieitong guo ji yin shui ping zhuai yi (HGT)er lai 。
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论文作者分别是来自山东农业大学的梁飞阳，发表于刊物山东农业大学2019-07-05论文，是一篇关于马铃薯疮痂病论文,系统鉴定论文,基因组学论文,致病岛论文,毒素合成基因论文,山东农业大学2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自山东农业大学2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：马铃薯疮痂病论文; 系统鉴定论文; 基因组学论文; 致病岛论文; 毒素合成基因论文; 山东农业大学2019-07-05论文;