：Molecular analyses of bacterioplankton communities with highly abundant Vibrio clades: a case study in Bohai Sea coastal waters论文

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作者（2019）在《Molecular analyses of bacterioplankton communities with highly abundant Vibrio clades: a case study in Bohai Sea coastal waters》一文中研究指出：We investigated the bacterioplankton abundance,community composition and the associated Vibrio clades of natural seawater in Bohai Sea coastal waters.Seawater samples(10 L in triplicate)were collected at 0.5,3,and 5 m depths near the coastal aquaculture zone of the Bohai Sea on May 12,2016.Real-time PCR and 16 S rRNA gene amplicon high-throughput sequencing methods were employed by which 485 operational taxonomic units(OTUs)at a 97% sequence similarity level were generated.Gammaproteobacteria,Alphaproteobacteria,and Bacteroidetes were the most abundant groups,accounting for 49.5%,23.5%,and 18.9% ofthe total assemblage,respectively.Obvious variations in Pseudoalteromonas,Vibrio,and Octadecabacter,which were the most abundant genera,could be observed among different samples.Notably,the results of Vibrio-specific real-time PCR indicated that Vibrio had extremely high 16 S rRNA gene copy numbers.The 16 S rRNA gene sequencing results across all the samples also indicated that they occupied a large proportion of the total assemblage.Both the alpha diversity and major bacterioplankton group Pseudoalteromonas had significant correlations with the concentration of PO43-.Overall,studies on bacterioplankton communities with highly abundant Vibrio clades can provide interesting insight into the microbial function and health assessment of the Bohai Sea coastal ecosystem.

Abstract

We investigated the bacterioplankton abundance,community composition and the associated Vibrio clades of natural seawater in Bohai Sea coastal waters.Seawater samples(10 L in triplicate)were collected at 0.5,3,and 5 m depths near the coastal aquaculture zone of the Bohai Sea on May 12,2016.Real-time PCR and 16 S rRNA gene amplicon high-throughput sequencing methods were employed by which 485 operational taxonomic units(OTUs)at a 97% sequence similarity level were generated.Gammaproteobacteria,Alphaproteobacteria,and Bacteroidetes were the most abundant groups,accounting for 49.5%,23.5%,and 18.9% ofthe total assemblage,respectively.Obvious variations in Pseudoalteromonas,Vibrio,and Octadecabacter,which were the most abundant genera,could be observed among different samples.Notably,the results of Vibrio-specific real-time PCR indicated that Vibrio had extremely high 16 S rRNA gene copy numbers.The 16 S rRNA gene sequencing results across all the samples also indicated that they occupied a large proportion of the total assemblage.Both the alpha diversity and major bacterioplankton group Pseudoalteromonas had significant correlations with the concentration of PO43-.Overall,studies on bacterioplankton communities with highly abundant Vibrio clades can provide interesting insight into the microbial function and health assessment of the Bohai Sea coastal ecosystem.

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