论文摘要
背景与目的:肿瘤的血管发生与肿瘤的生长关系密切。在肿瘤血管发生的分子机制的研究中,DLL4的作用日益受到关注。研究认为,肿瘤血管内皮细胞高表达DLL4,DLL4可能在血管的生长过程中调节着血管内皮细胞的分化增殖,从而达到控制血管生长的作用。本实验拟检测血管内皮细胞是否表达DLL4以及DLL4的两个下游基因HEY1和HEY2。研究DLL4对血管内皮细胞增殖的影响,探索DLL4在肿瘤血管发生和生长中的作用。材料与方法:培养人微血管内皮细胞（HMEC）,MTT法检测HMEC细胞增殖,提取HMEC细胞总RNA,用RT-PCR的方法检测HMEC细胞DLL4mRNA、HEY1mRNA、HEY2mRNA的表达,采用RNA干扰方法观察HMEC细胞DLL4 mRNA表达,对结果进行统计学分析。结果:RT-PCR结果显示,HMEC细胞表达DLL4 mRNA、HEY1 mRNA和HEY2 mRNA;DLL4 RNA干扰结果显示,100nmol/L siRNA DLL4-duplex组和200nmol/L siRNA DLL4-duplex组HMEC细胞DLL4 mRNA的表达明显下调,MTT的结果显示100nmol/L siRNA DLL4-duplex组和200nmol/L siRNA DLL4-duplex组的HMEC细胞的吸光度明显增加,即抑制DLL4能促进HMEC细胞的增殖,表明DLL4抑制HMEC细胞增殖。结论: HMEC细胞表达DLL4 mRNA、HEY1 mRNA和HEY2 mRNA; DLL4抑制HMEC细胞增殖。
论文目录
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