论文摘要
目的 瘦素(Leptin,LEP)是脂肪细胞分泌的小分子肽类物质,研究表明它不仅与能量代谢有关,还具有其它许多生理功能。由于肥胖能导致许多疾病发生,肥胖者体内Leptin在其中所起的作用需要进行深入探讨。本研究通过检测肥胖儿童血液中Leptin的含量,观察Leptin与体内其它能量代谢因子和青春期发育激素的关系,并采用抑制性消减杂交(Suppression Subtractive Hybridization,SSH)技术探讨Leptin诱导人胚肾293(HEK293)细胞后基因的差异表达情况,从人体激素变化和细胞基因表达二个方面探讨Leptin对机体的作用及其机制。 方法 1.选择12~14岁肥胖儿童99例和正常对照儿童105例为研究对象,采用放射免疫法分别测定血中的瘦素(Leptin,LEP)、食欲素(Orexins)、神经肽Y(Neuropeptide Y,NPY)、胰岛素(Insulin,Ins)C—肽(C—peptide)浓度和睾酮(Testosterone,T)、雌二醇(Estradiol,E2)、卵泡刺激素(Follicle stimulating hormone,FSH)、黄体生成素(Luteotrophic hormone,LH)等浓度,采用酶联免疫吸附分析法测定可溶性瘦素受体(Soluble leptin receptor,sOB-R)。 1)采用分组对比分析和相关回归法来探讨Leptin在青春期肥胖儿童体内的变化和对肥胖儿童体内能量代谢因子的影响。 2)采用分组对比分析和相关分析法探讨Leptin对肥胖儿童青春期性发育激素的影响。
论文目录
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标签:瘦素论文; 食欲素论文; 神经肽论文; 胰岛素论文; 睾酮论文; 雌二醇论文; 黄体生成素论文; 卵泡刺激素论文; 文库论文; 抑制性消减杂交论文; 斑点杂交论文; 青春期论文; 肥胖论文;