本文主要研究内容
作者冯玉龙（2019）在《H2O2调控盐胁迫下番茄幼苗生长的研究》一文中研究指出：番茄作为中度盐敏感型蔬菜,设施土壤次生盐渍化严重限制设施种植条件下番茄植株生长和产量形成。因此,开展提高番茄盐适应性的研究具有重要意义。前人及前期研究表明,过氧化氢（H2O2）作为一种信号分子能增强植物的抗逆性。因此,本文以番茄“中蔬四号”品种的幼苗为试验材料,采用药理学手段,通过对NaCl胁迫下番茄幼苗叶片分别外源施用H2O2（N+H处理）、DMTU（N+D处理）和H2O2+DMTU（N+H+D处理）来构建不同的内源H2O2水平。研究外源H2O2介导内源H2O2水平对盐胁迫下番茄幼苗生长、渗透调节、抗氧化酶促系统、光合特性的影响,以揭示H2O2在植物抗盐胁迫反应中的作用机制,为进一步阐明番茄耐盐性形成机制提供理论依据。主要结论如下:1.与对照相比,NaCl处理显著降低了番茄幼苗叶绿素含量和根系活力,抑制植株的生长,减少了生物量的积累;NaCl胁迫条件下外源DMTU、H2O2和H2O2+DMTU均能显著降低内源H2O2水平、提高番茄幼苗叶绿素含量和根系活力,促进植株的生长,增加了生物量的积累,但外源施用DMTU和H2O2+DMTU对NaCl胁迫下对番茄幼生长的促进作用及内源H2O2水平均显著低于施用H2O2的。说明在NaCl胁迫下通过叶面喷施DMTU、H2O2和H2O2+DMTU介导低水平的内源H2O2可显著促进NaCl胁迫下番茄幼苗生长,但外源H2O2介导番茄幼苗维持一定较高水平的内源H2O2更有利于促进生长,提高番茄的盐适应性。2.NaCl胁迫显著增加了番茄幼苗MDA含量,提高了电解质渗透率,加剧膜脂过氧化程度,破坏细胞膜结构,从而抑制番茄幼苗的生长;NaCl胁迫条件下,叶面喷施DMTU、H2O2和H2O2+DMTU均使MDA含量和电解质渗透率降低。此外Pro含量增加。说明在NaCl胁迫下叶面喷施DMTU、H2O2和H2O2+DMTU通过调控降低内源H2O2水平显著缓解NaCl胁迫导致的膜脂过氧化,维护膜的完整性和维持细胞渗透压平衡,从而提高番茄的盐适应性,且外源H2O2介导番茄幼苗维持一定较高水平的内源H2O2能更好缓解NaCl胁迫对番茄幼细胞膜的损伤及诱导渗透调节提高。3.NaCl胁迫下调了番茄幼苗叶片SOD、CAT、POD和AsA-GSH循环关键抗氧化酶活性,从而导致H2O2累积,促进膜脂过氧化,使番茄幼苗受到严重的氧化胁迫;在NaCl胁迫下,番茄幼苗叶面喷施DMTU、H2O2、H2O2+DMTU均可不同程度上调SOD、CAT、POD和AsA-GSH循环关键抗氧化酶活性,降低内源H2O2水平,说明叶面喷施DMTU、H2O2、H2O2+DMTU可不同程度缓解NaCl胁迫对番茄幼苗的氧化胁迫,且喷施H2O2比喷施DMTU和H2O2+DMTU能更好的缓解NaCl胁迫下造成的氧化胁迫对番茄幼苗的伤害。4.NaCl胁迫显著降低了番茄幼苗光合参数Pn、Tr、Ci、Gs,提高了VPD和WUE,显著抑制了荧光参数Fv/Fm、Y（II）、qP,提高了qN和Y（NPQ）值,说明NaCl胁迫导致番茄幼苗Pn下降主要以气孔限制因素为主,且番茄幼苗通过热耗散的方式来消耗PSII的过多光能,以适应盐胁迫环境;而NaCl胁迫下番茄幼苗叶面喷施DMTU、H2O2、H2O2+DMTU可不同程度通过提高胞间光合底物（CO2）浓度及传导能力、促进PSII光化学反应活性和利用效率、激发能量热耗散以保护光合机构免受损伤等气孔和非气孔限制因素来缓解NaCl胁迫对番茄幼苗光合作用的抑制作用。且外源喷施H2O2比喷施DMTU和H2O2+DMTU能更好的缓解NaCl胁迫对番茄幼苗光合作用的抑制作用。
Abstract
fan jia zuo wei zhong du yan min gan xing shu cai ,she shi tu rang ci sheng yan zi hua yan chong xian zhi she shi chong zhi tiao jian xia fan jia zhi zhu sheng chang he chan liang xing cheng 。yin ci ,kai zhan di gao fan jia yan kuo ying xing de yan jiu ju you chong yao yi yi 。qian ren ji qian ji yan jiu biao ming ,guo yang hua qing （H2O2）zuo wei yi chong xin hao fen zi neng zeng jiang zhi wu de kang ni xing 。yin ci ,ben wen yi fan jia “zhong shu si hao ”pin chong de you miao wei shi yan cai liao ,cai yong yao li xue shou duan ,tong guo dui NaClxie pai xia fan jia you miao xie pian fen bie wai yuan shi yong H2O2（N+Hchu li ）、DMTU（N+Dchu li ）he H2O2+DMTU（N+H+Dchu li ）lai gou jian bu tong de nei yuan H2O2shui ping 。yan jiu wai yuan H2O2jie dao nei yuan H2O2shui ping dui yan xie pai xia fan jia you miao sheng chang 、shen tou diao jie 、kang yang hua mei cu ji tong 、guang ge te xing de ying xiang ,yi jie shi H2O2zai zhi wu kang yan xie pai fan ying zhong de zuo yong ji zhi ,wei jin yi bu chan ming fan jia nai yan xing xing cheng ji zhi di gong li lun yi ju 。zhu yao jie lun ru xia :1.yu dui zhao xiang bi ,NaClchu li xian zhe jiang di le fan jia you miao xie lu su han liang he gen ji huo li ,yi zhi zhi zhu de sheng chang ,jian shao le sheng wu liang de ji lei ;NaClxie pai tiao jian xia wai yuan DMTU、H2O2he H2O2+DMTUjun neng xian zhe jiang di nei yuan H2O2shui ping 、di gao fan jia you miao xie lu su han liang he gen ji huo li ,cu jin zhi zhu de sheng chang ,zeng jia le sheng wu liang de ji lei ,dan wai yuan shi yong DMTUhe H2O2+DMTUdui NaClxie pai xia dui fan jia you sheng chang de cu jin zuo yong ji nei yuan H2O2shui ping jun xian zhe di yu shi yong H2O2de 。shui ming zai NaClxie pai xia tong guo xie mian pen shi DMTU、H2O2he H2O2+DMTUjie dao di shui ping de nei yuan H2O2ke xian zhe cu jin NaClxie pai xia fan jia you miao sheng chang ,dan wai yuan H2O2jie dao fan jia you miao wei chi yi ding jiao gao shui ping de nei yuan H2O2geng you li yu cu jin sheng chang ,di gao fan jia de yan kuo ying xing 。2.NaClxie pai xian zhe zeng jia le fan jia you miao MDAhan liang ,di gao le dian jie zhi shen tou lv ,jia ju mo zhi guo yang hua cheng du ,po huai xi bao mo jie gou ,cong er yi zhi fan jia you miao de sheng chang ;NaClxie pai tiao jian xia ,xie mian pen shi DMTU、H2O2he H2O2+DMTUjun shi MDAhan liang he dian jie zhi shen tou lv jiang di 。ci wai Prohan liang zeng jia 。shui ming zai NaClxie pai xia xie mian pen shi DMTU、H2O2he H2O2+DMTUtong guo diao kong jiang di nei yuan H2O2shui ping xian zhe huan jie NaClxie pai dao zhi de mo zhi guo yang hua ,wei hu mo de wan zheng xing he wei chi xi bao shen tou ya ping heng ,cong er di gao fan jia de yan kuo ying xing ,ju wai yuan H2O2jie dao fan jia you miao wei chi yi ding jiao gao shui ping de nei yuan H2O2neng geng hao huan jie NaClxie pai dui fan jia you xi bao mo de sun shang ji you dao shen tou diao jie di gao 。3.NaClxie pai xia diao le fan jia you miao xie pian SOD、CAT、PODhe AsA-GSHxun huan guan jian kang yang hua mei huo xing ,cong er dao zhi H2O2lei ji ,cu jin mo zhi guo yang hua ,shi fan jia you miao shou dao yan chong de yang hua xie pai ;zai NaClxie pai xia ,fan jia you miao xie mian pen shi DMTU、H2O2、H2O2+DMTUjun ke bu tong cheng du shang diao SOD、CAT、PODhe AsA-GSHxun huan guan jian kang yang hua mei huo xing ,jiang di nei yuan H2O2shui ping ,shui ming xie mian pen shi DMTU、H2O2、H2O2+DMTUke bu tong cheng du huan jie NaClxie pai dui fan jia you miao de yang hua xie pai ,ju pen shi H2O2bi pen shi DMTUhe H2O2+DMTUneng geng hao de huan jie NaClxie pai xia zao cheng de yang hua xie pai dui fan jia you miao de shang hai 。4.NaClxie pai xian zhe jiang di le fan jia you miao guang ge can shu Pn、Tr、Ci、Gs,di gao le VPDhe WUE,xian zhe yi zhi le ying guang can shu Fv/Fm、Y（II）、qP,di gao le qNhe Y（NPQ）zhi ,shui ming NaClxie pai dao zhi fan jia you miao Pnxia jiang zhu yao yi qi kong xian zhi yin su wei zhu ,ju fan jia you miao tong guo re hao san de fang shi lai xiao hao PSIIde guo duo guang neng ,yi kuo ying yan xie pai huan jing ;er NaClxie pai xia fan jia you miao xie mian pen shi DMTU、H2O2、H2O2+DMTUke bu tong cheng du tong guo di gao bao jian guang ge de wu （CO2）nong du ji chuan dao neng li 、cu jin PSIIguang hua xue fan ying huo xing he li yong xiao lv 、ji fa neng liang re hao san yi bao hu guang ge ji gou mian shou sun shang deng qi kong he fei qi kong xian zhi yin su lai huan jie NaClxie pai dui fan jia you miao guang ge zuo yong de yi zhi zuo yong 。ju wai yuan pen shi H2O2bi pen shi DMTUhe H2O2+DMTUneng geng hao de huan jie NaClxie pai dui fan jia you miao guang ge zuo yong de yi zhi zuo yong 。
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论文作者分别是来自石河子大学的冯玉龙，发表于刊物石河子大学2019-09-26论文，是一篇关于番茄论文,胁迫论文,抗氧化系统论文,光合特性论文,石河子大学2019-09-26论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自石河子大学2019-09-26论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：番茄论文; 胁迫论文; 抗氧化系统论文; 光合特性论文; 石河子大学2019-09-26论文;