本文主要研究内容
作者杜鑫哲（2019）在《H2S信号与MAPK在拟南芥应答干旱与冷胁迫中的关系》一文中研究指出：硫化氢（hydrogen sulfide,H2S）是继一氧化氮（nitric oxide,NO）与一氧化碳（carbon oxide,CO）之后的第三种气体信号分子,在动植物体内可以内源性地产生,而且发挥着重要的生理功能。在植物中,H2S调控生长发育以及对生物胁迫和非生物胁迫的响应等生理过程。H2S能够增加植物对干旱胁迫与冷胁迫的抵御能力,然而其深入的分子机制还未完全得到阐述。促分裂原活化蛋白激酶（mitogen-activated protein kinase,MAPK）是真核生物中保守存在的信号转导组分,能够传递生长发育信号以及环境刺激信号从而引起细胞响应,在植物响应干旱胁迫和冷胁迫的过程中发挥着重要的作用。然而在植物中H2S与MAPK之间的关系还未得到揭示。本研究以拟南芥为材料,探索了干旱胁迫与冷胁迫下H2S与MAPK之间的关系,主要结果如下:1.干旱胁迫诱导了内源H2S的生成以及MAPK的基因表达,而在H2S生成关键酶编码基因缺失的双突变体lcd/des1中MAPK基因表达量在干旱胁迫后下降。NaHS（H2S供体）处理上调了MAPK基因尤其是MPK4的表达水平,所以选择MPK4为主要研究对象并获得了mpk4突变体用于随后的研究。在WT中H2S能够缓解干旱胁迫造成的损伤,而在mpk4中H2S对干旱胁迫的缓解作用被抑制。H2S对气孔运动的调控过程需要MPK4的参与。随后我们检测了H2S与MPK4在脱落酸（abscisic acid,ABA）与过氧化氢（hydrogen peroxide,H2O2）调控的气孔运动过程中的作用,发现ABA与H2O2介导的气孔运动在lcd/des1与mpk4中均受到抑制。H2S诱导的气孔关闭过程在slac1-3中被抑制。以上结果说明在干旱胁迫响应以及气孔运动过程中MPK4是H2S的下游组分,而且H2S-MPK4级联参与了ABA调控的气孔运动过程来缓解干旱胁迫。2.在H2S生成关键酶编码基因缺失的单突变体des1与lcd中,MAPK的基因表达量降低。冷胁迫对MAPK基因表达的诱导作用在des1与lcd中被抑制。H2S缓解冷胁迫的能力在mpk4中被抑制。H2S与MPK4能够调控冷胁迫响应基因ICE1（inducer of CBF expression 1）、CBF3（C-repeat-binding factors）、COR15A（cold responsive 15A）与COR15B（cold responsive 15B）的表达。H2S抑制了冷胁迫下的气孔张开过程,而这个作用在mpk4中被抑制。以上结果说明在H2S缓解冷胁迫的过程中,MPK4是重要的下游组分,H2S与MPK4通过调节冷胁迫响应基因的表达以及气孔运动来帮助植物抵御冷胁迫。3.mek2突变体中H2S缓解冷胁迫的能力没有受到影响。MEK2蛋白不能发生巯基化修饰。MPK4蛋白有本底巯基化信号,而且NaHS处理能够增加MPK4的巯基化水平。以上结果说明在H2S缓解冷胁迫的过程中不通过MEK2而是通过巯基化修饰直接作用于MPK4发挥作用。4.NaHS处理能够上调生物钟相关基因CCA1（circadian clock associated 1）和PRR9（pseudo-response regulator 9）的表达,而且在lcd/des1中,CCA1与PRR9的峰值表达时间明显滞后。CBFs（C-repeat binding factors）是受CCA1调控的冷胁迫响应基因,其表达也受H2S的调控,lcd/des1中CBF1和CBF3的峰值表达时间延迟,同时在lcd/des1中CBF1、CBF2和CBF3都下调表达。lcd/des1幼苗对冷胁迫表现出更高的敏感性。对拟南芥内源H2S生成关键酶编码基因LCD（L-cysteine desulfhydrase）与DES1（desulfhydrase 1）的节律性进行了初步的探索发现,LCD的表达在1 d内没有明显的变化,而DES1的表达有明显的节律性,在早上8:00达到峰值。在lcd/des1中,MPK3、MPK4与MPK6的昼夜表达模式发生改变。以上结果说明H2S能够通过调节CCA1与PRR9基因的表达调控生物钟,进而影响下游靶标CBFs基因的表达以增加拟南芥对冷胁迫的耐受性。综上,我们发现在拟南芥响应干旱胁迫与冷胁迫的过程中,H2S与MAPK之间存在紧密的关系,MAPK家族的成员MPK4是H2S的重要下游组分,H2S能够直接巯基化修饰MPK4来发挥生理作用,帮助植物增加对干旱胁迫与冷胁迫的抗性。
Abstract
liu hua qing （hydrogen sulfide,H2S）shi ji yi yang hua dan （nitric oxide,NO）yu yi yang hua tan （carbon oxide,CO）zhi hou de di san chong qi ti xin hao fen zi ,zai dong zhi wu ti nei ke yi nei yuan xing de chan sheng ,er ju fa hui zhao chong yao de sheng li gong neng 。zai zhi wu zhong ,H2Sdiao kong sheng chang fa yo yi ji dui sheng wu xie pai he fei sheng wu xie pai de xiang ying deng sheng li guo cheng 。H2Sneng gou zeng jia zhi wu dui gan han xie pai yu leng xie pai de di yu neng li ,ran er ji shen ru de fen zi ji zhi hai wei wan quan de dao chan shu 。cu fen lie yuan huo hua dan bai ji mei （mitogen-activated protein kinase,MAPK）shi zhen he sheng wu zhong bao shou cun zai de xin hao zhuai dao zu fen ,neng gou chuan di sheng chang fa yo xin hao yi ji huan jing ci ji xin hao cong er yin qi xi bao xiang ying ,zai zhi wu xiang ying gan han xie pai he leng xie pai de guo cheng zhong fa hui zhao chong yao de zuo yong 。ran er zai zhi wu zhong H2Syu MAPKzhi jian de guan ji hai wei de dao jie shi 。ben yan jiu yi ni na gai wei cai liao ,tan suo le gan han xie pai yu leng xie pai xia H2Syu MAPKzhi jian de guan ji ,zhu yao jie guo ru xia :1.gan han xie pai you dao le nei yuan H2Sde sheng cheng yi ji MAPKde ji yin biao da ,er zai H2Ssheng cheng guan jian mei bian ma ji yin que shi de shuang tu bian ti lcd/des1zhong MAPKji yin biao da liang zai gan han xie pai hou xia jiang 。NaHS（H2Sgong ti ）chu li shang diao le MAPKji yin you ji shi MPK4de biao da shui ping ,suo yi shua ze MPK4wei zhu yao yan jiu dui xiang bing huo de le mpk4tu bian ti yong yu sui hou de yan jiu 。zai WTzhong H2Sneng gou huan jie gan han xie pai zao cheng de sun shang ,er zai mpk4zhong H2Sdui gan han xie pai de huan jie zuo yong bei yi zhi 。H2Sdui qi kong yun dong de diao kong guo cheng xu yao MPK4de can yu 。sui hou wo men jian ce le H2Syu MPK4zai tuo la suan （abscisic acid,ABA）yu guo yang hua qing （hydrogen peroxide,H2O2）diao kong de qi kong yun dong guo cheng zhong de zuo yong ,fa xian ABAyu H2O2jie dao de qi kong yun dong zai lcd/des1yu mpk4zhong jun shou dao yi zhi 。H2Syou dao de qi kong guan bi guo cheng zai slac1-3zhong bei yi zhi 。yi shang jie guo shui ming zai gan han xie pai xiang ying yi ji qi kong yun dong guo cheng zhong MPK4shi H2Sde xia you zu fen ,er ju H2S-MPK4ji lian can yu le ABAdiao kong de qi kong yun dong guo cheng lai huan jie gan han xie pai 。2.zai H2Ssheng cheng guan jian mei bian ma ji yin que shi de chan tu bian ti des1yu lcdzhong ,MAPKde ji yin biao da liang jiang di 。leng xie pai dui MAPKji yin biao da de you dao zuo yong zai des1yu lcdzhong bei yi zhi 。H2Shuan jie leng xie pai de neng li zai mpk4zhong bei yi zhi 。H2Syu MPK4neng gou diao kong leng xie pai xiang ying ji yin ICE1（inducer of CBF expression 1）、CBF3（C-repeat-binding factors）、COR15A（cold responsive 15A）yu COR15B（cold responsive 15B）de biao da 。H2Syi zhi le leng xie pai xia de qi kong zhang kai guo cheng ,er zhe ge zuo yong zai mpk4zhong bei yi zhi 。yi shang jie guo shui ming zai H2Shuan jie leng xie pai de guo cheng zhong ,MPK4shi chong yao de xia you zu fen ,H2Syu MPK4tong guo diao jie leng xie pai xiang ying ji yin de biao da yi ji qi kong yun dong lai bang zhu zhi wu di yu leng xie pai 。3.mek2tu bian ti zhong H2Shuan jie leng xie pai de neng li mei you shou dao ying xiang 。MEK2dan bai bu neng fa sheng qiu ji hua xiu shi 。MPK4dan bai you ben de qiu ji hua xin hao ,er ju NaHSchu li neng gou zeng jia MPK4de qiu ji hua shui ping 。yi shang jie guo shui ming zai H2Shuan jie leng xie pai de guo cheng zhong bu tong guo MEK2er shi tong guo qiu ji hua xiu shi zhi jie zuo yong yu MPK4fa hui zuo yong 。4.NaHSchu li neng gou shang diao sheng wu zhong xiang guan ji yin CCA1（circadian clock associated 1）he PRR9（pseudo-response regulator 9）de biao da ,er ju zai lcd/des1zhong ,CCA1yu PRR9de feng zhi biao da shi jian ming xian zhi hou 。CBFs（C-repeat binding factors）shi shou CCA1diao kong de leng xie pai xiang ying ji yin ,ji biao da ye shou H2Sde diao kong ,lcd/des1zhong CBF1he CBF3de feng zhi biao da shi jian yan chi ,tong shi zai lcd/des1zhong CBF1、CBF2he CBF3dou xia diao biao da 。lcd/des1you miao dui leng xie pai biao xian chu geng gao de min gan xing 。dui ni na gai nei yuan H2Ssheng cheng guan jian mei bian ma ji yin LCD（L-cysteine desulfhydrase）yu DES1（desulfhydrase 1）de jie lv xing jin hang le chu bu de tan suo fa xian ,LCDde biao da zai 1 dnei mei you ming xian de bian hua ,er DES1de biao da you ming xian de jie lv xing ,zai zao shang 8:00da dao feng zhi 。zai lcd/des1zhong ,MPK3、MPK4yu MPK6de zhou ye biao da mo shi fa sheng gai bian 。yi shang jie guo shui ming H2Sneng gou tong guo diao jie CCA1yu PRR9ji yin de biao da diao kong sheng wu zhong ,jin er ying xiang xia you ba biao CBFsji yin de biao da yi zeng jia ni na gai dui leng xie pai de nai shou xing 。zeng shang ,wo men fa xian zai ni na gai xiang ying gan han xie pai yu leng xie pai de guo cheng zhong ,H2Syu MAPKzhi jian cun zai jin mi de guan ji ,MAPKjia zu de cheng yuan MPK4shi H2Sde chong yao xia you zu fen ,H2Sneng gou zhi jie qiu ji hua xiu shi MPK4lai fa hui sheng li zuo yong ,bang zhu zhi wu zeng jia dui gan han xie pai yu leng xie pai de kang xing 。
论文参考文献
论文详细介绍
论文作者分别是来自山西大学的杜鑫哲，发表于刊物山西大学2019-11-12论文，是一篇关于硫化氢论文,干旱胁迫论文,冷胁迫论文,气孔论文,山西大学2019-11-12论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自山西大学2019-11-12论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：硫化氢论文; 干旱胁迫论文; 冷胁迫论文; 气孔论文; 山西大学2019-11-12论文;